Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inducible transgene expression in mouse stem cells.

David T Ting1, Michael Kyba, George Q Daley

  • 1Harvard-M.I.T. Program in Health Sciences and Technology, Harvard Medical School. Boston, MA, USA.

Methods in Molecular Medicine
|October 20, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic monitoring of antibody drug conjugates targeting TROP2 or HER2 in breast cancer using circulating tumor cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

B7-H3 CAR T cells eradicate intrahepatic cholangiocarcinoma and induce durable response.

Journal of experimental & clinical cancer research : CR·2026
Same author

Neoadjuvant stereotactic body radiation therapy plus immune therapy favorably remodels the hepatocellular carcinoma tumor microenvironment.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Author Correction: HER2 expression identifies dynamic functional states within circulating breast cancer cells.

Nature·2026
Same author

TROP2/claudin program mediates immune exclusion to impede checkpoint blockade in breast cancer.

Journal for immunotherapy of cancer·2026
Same author

Disparities in the diagnosis and management of exocrine pancreatic insufficiency in resectable vs metastatic pancreatic cancer.

The oncologist·2026

Researchers developed a doxycycline-inducible system for precise control over gene expression in embryonic stem (ES) cells. This system enables the development of specific cell types, like hematopoietic stem cells, for potential disease treatments.

Area of Science:

  • Stem cell biology
  • Gene expression regulation
  • Regenerative medicine

Background:

  • Embryonic stem (ES) cells offer a potential source for cell and tissue therapies.
  • Current methods for manipulating ES cell differentiation lack precise control over gene expression.
  • Spatial, temporal, and quantitative control are crucial for studying developmental genes.

Purpose of the Study:

  • To address limitations in controlling gene expression during ES cell differentiation.
  • To introduce and characterize a doxycycline-inducible transgene system in ES cells.
  • To demonstrate the system's utility in generating specific cell types for therapeutic applications.

Main Methods:

  • Development of a doxycycline-inducible transgene system in an ES cell line.

Related Experiment Videos

  • Characterization of the inducible transgene system's performance.
  • Application of the system to derive ES-derived hematopoietic stem cells.

    • Main Results:

    • Successful establishment of a doxycycline-inducible system in ES cells.
    • Demonstration of controlled gene expression for ES cell differentiation.
    • Generation of ES-derived long-term engrafting hematopoietic stem cells.

    Conclusions:

    • The doxycycline-inducible system provides robust spatial, temporal, and quantitative control over gene expression in ES cells.
    • This versatile system facilitates the development of specific cell lineages, such as hematopoietic stem cells.
    • The system holds significant potential for applications in regenerative medicine and disease modeling.