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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Published on: March 12, 2017
Nucleotide sequence analysis of human tpr cDNA clones
1Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK.
Oncogene
|February 1, 1992
Summary
Researchers characterized the tpr gene, involved in proto-oncogene activation. Alternative splicing creates tpr variants with extended domains, suggesting roles in cell regulation and potential cancer pathways.
Area of Science:
- Molecular Biology
- Oncogenesis
Background:
- The tpr gene is implicated in the activation of met and raf proto-oncogenes.
- Understanding tpr's function is crucial for oncogenesis research.
Purpose of the Study:
- To characterize cDNA clones of the tpr gene.
- To investigate alternative splicing events affecting tpr mRNA and protein structure.
Main Methods:
- Sequencing of tpr cDNA clones from a human fibrosarcoma cell line.
- Analysis of open reading frame (ORF) and alternative splicing.
- Bioinformatic prediction of protein structure and homology.
Main Results:
- Identified a 726 amino acid tpr ORF.
- Discovered alternative splicing leading to an extended C-terminal domain.
- Predicted extensive alpha-helix regions and coiled-coil motifs in the tpr protein.
- Observed weak homology to tropomyosin, spectrin, and other coiled-coil proteins.
Conclusions:
- Alternative splicing generates tpr protein variants with altered C-terminal domains.
- The tpr protein's structure suggests involvement in protein-protein interactions.
- Further research is needed to elucidate tpr's precise role in proto-oncogene activation and cancer.
