In vitro testing methodologies. Evolution and current status

Almost 25 years after the development of the radioallergosorbent test, in vitro technology has become firmly established as an outstanding diagnostic tool for physicians treating the allergic patient. The American Academy of Otolaryngic Allergy (AAO), in its most current position statement, endorses the modified RAST as reproducible, sensitive, and specific. A new organization called the American In Vitro Allergy and Immunology Society (AIVAIS) has been formed to promote and monitor the use of these tests. Both societies suggest that in vitro tests should be part of a complete clinical evaluation by a well informed physician knowledgeable about the contraindications and limitations of in vitro technology. Such physicians should be capable of incorporating this information into responsible, judicious, and effective management of allergic patients. Only after the correct diagnosis has been made can appropriate treatment be started. Whichever route you choose, you and your patients can benefit from the use of in vitro allergy testing.