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Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
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Single protein molecular dynamics determined with ultra-high precision

Y C Sasaki1

  • 1Bio-medical Group, SPring-8/Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Mikazuki-cho, Sayou-gun, Hyougo 679-5198, Japan.

Biochemical Society Transactions
|October 21, 2004
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...