Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Plakoglobin expression and localization in zebrafish embryo development.

E D Martin¹, M Grealy

¹Pharmacology Department and National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland. eva.martin@nuigalway.ie

Biochemical Society Transactions

|October 21, 2004

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

p38 MAPK in cardioprotection - are we there yet?

British journal of pharmacology·2014

Same author

Dopamine D(1) receptor deletion strongly reduces neurotoxic effects of methamphetamine.

Neurobiology of disease·2011

Same author

Brain-derived neurotrophic factor modulates the severity of cognitive alterations induced by mutant huntingtin: involvement of phospholipaseCgamma activity and glutamate receptor expression.

Neuroscience·2009

Same author

Anxiety sensitivity and drug or alcohol use in individuals with anxiety and substance use disorders.

Addictive behaviors·2002

Same author

Synaptic regulation of the slow Ca2+-activated K+ current in hippocampal CA1 pyramidal neurons: implication in epileptogenesis.

Journal of neurophysiology·2001

Same author

Effect of adenylyl cyclase activation on intracellular and extracellular cAMP and cGMP in preimplantation cattle blastocysts.

Journal of reproduction and fertility·2000

Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026

Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026

Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026

Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026

Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026

Same journal

Implications of Rho GTPase signaling in cancer immunotherapy.

Biochemical Society transactions·2026

See all related articles

Plakoglobin, a key cell adhesion protein, shifts its location during zebrafish heart development. Its expression increases and localization changes from adherens junctions to desmosomes by 72 hours post-fertilization.

Area of Science:

Cell Biology
Developmental Biology
Molecular Biology

Background:

Plakoglobin (gamma-catenin) and beta-catenin are crucial for cell-cell adhesion at adherens junctions.
These proteins can translocate to the nucleus upon Wnt signaling pathway activation.
Plakoglobin is also a component of desmosomes, specialized cell adhesion structures found in vertebrates.

Purpose of the Study:

To investigate the expression and localization patterns of plakoglobin during zebrafish embryonic development.
To understand the dynamic changes in plakoglobin's cellular location in relation to beta-catenin.

Main Methods:

Western blotting was employed to quantify plakoglobin protein levels throughout zebrafish embryonic development.
Confocal microscopy was utilized to visualize the subcellular localization of plakoglobin and beta-catenin.

Related Experiment Videos

Main Results:

Plakoglobin protein was detected throughout zebrafish embryonic development, with increased content observed between 12 and 24 hours post-fertilization (hpf).
Initially (up to 12 hpf), plakoglobin and beta-catenin were co-localized in both the nucleus and cell-cell junctions.
By 24 and 72 hpf, distinct localization patterns emerged, with plakoglobin shifting from adherens junctions to desmosomes in the developing heart region.

Conclusions:

Plakoglobin exhibits dynamic expression and localization changes during zebrafish embryogenesis.
The observed shift in plakoglobin localization from adherens junctions to desmosomes correlates with heart chamber development.
These findings highlight the differential roles of plakoglobin and beta-catenin in establishing cell adhesion structures during vertebrate development.