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Updated: Aug 21, 2026
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
Published on: May 10, 2022
Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay
Galina G Hovhannisyan1, Tigran S Haroutunyan, Rouben M Arutyunyan
1Department of Genetics and Cytology, Yerevan State University, Yerevan 375049, Armenia.
Unlabelled:
The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors.
Methods:
DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The RESULTS obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration.
Conclusion:
The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.
