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Related Experiment Videos

Three-dimensional structure of Theiler virus.

R A Grant1, D J Filman, R S Fujinami

  • 1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

Proceedings of the National Academy of Sciences of the United States of America
|March 15, 1992
PubMed
Summary

Theiler virus strains differ in neurovirulence. Structural analysis of the demyelinating strain reveals unique features on its surface, potentially explaining differences in disease progression and host interaction.

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Area of Science:

  • Virology
  • Structural Biology
  • Neuroscience

Background:

  • Theiler murine encephalomyelitis virus (TMEV) exists in neurovirulent and demyelinating forms.
  • Demyelinating TMEV causes motor neuron infection and white matter lesions resembling multiple sclerosis.
  • Understanding TMEV structure is key to deciphering its pathogenesis.

Purpose of the Study:

  • To determine the three-dimensional structure of the demyelinating TMEV DA strain.
  • To identify structural features that may correlate with differences in viral pathogenesis.
  • To investigate potential mechanisms of viral recognition and host interaction.

Main Methods:

  • X-ray crystallography at 2.8 Å resolution.
  • Analysis of the icosahedral capsid structure.

Related Experiment Videos

  • Comparison of surface features with known picornaviruses.

    • Main Results:

    • The DA strain capsid is composed of wedge-shaped beta barrels, typical of picornaviruses.
    • Unique surface features include star-shaped plateaus and depressions, with unusual loops and a cysteine-rich loop.
    • Sequence differences between demyelinating and neurovirulent strains are located on the surface plateau, suggesting roles in host recognition.

    Conclusions:

    • The determined structure provides a basis for understanding TMEV's demyelinating potential.
    • Specific surface structures and sequence variations likely mediate host-pathogen interactions.
    • These findings offer insights into the molecular basis of TMEV-induced demyelination and potential therapeutic targets.