Related Experiment Video

Updated: Aug 3, 2026

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

Accumulation of cell wall hydroxyproline-rich glycoprotein mRNA is an early event in maize embryo cell

L Ruiz-Avila1, S R Burgess, V Stiefel

  • 1Departamento de Genètica Molecular, Desarrollo Consejo Superior de Investigaciones Cientificas, Barcelona, Spain.

Proceedings of the National Academy of Sciences of the United States of America
|March 15, 1992
PubMed

Insights

Hydroxyproline-rich glycoprotein (HRGP) mRNA accumulation in maize embryos marks early vascular system formation. This finding suggests HRGP mRNA is a marker for cell wall development before cellulose deposition.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Hydroxyproline-rich glycoproteins (HRGPs) are key components of plant cell walls in vegetative tissues.
  • Understanding the spatial and temporal regulation of HRGP gene expression is crucial for plant development.

Purpose of the Study:

  • To investigate the accumulation patterns of hydroxyproline-rich glycoprotein (HRGP) mRNA in maize embryos.
  • To identify potential roles of HRGP mRNA as a marker for vascular system development and cell wall formation.

Main Methods:

  • In situ hybridization was used to detect HRGP mRNA localization within maize embryos.
  • Histone H4 mRNA distribution was also analyzed for comparative cell cycle studies.

Main Results:

  • HRGP mRNA accumulates in the maize embryo axis, particularly in dividing and provascular cells, but not in the scutellum.
  • Histone H4 mRNA shows a similar but more restricted distribution, indicating differential cell cycle accumulation.
  • HRGP mRNA accumulation correlates with cells having low cellulose content, suggesting its role in early cell wall formation.

Conclusions:

  • HRGP mRNA serves as a valuable marker for the early stages of vascular system development in maize embryos.
  • The observed mRNA accumulation pattern provides insights into the regulation of cell wall formation and cellulose deposition during embryogenesis.

More Related Videos

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging06:11

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Morphogenesis02:19

Morphogenesis

Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
The Phragmoplast01:59

The Phragmoplast

Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...