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Updated: Aug 21, 2026

Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
Published on: March 21, 2025
Genetic divergence and dispersal of yellow fever virus, Brazil
Pedro F C Vasconcelos1, Juliet E Bryant, Travassos P A da Rosa
1World Health Organization Collaborating Center for Arbovirus Reference and Research, Instituto Evandro Chagas, Belém, Brazil. pedrovasconcelos@iec.pa.gov.br
Abstract:
An analysis of 79 yellow fever virus (YFV) isolates collected from 1935 to 2001 in Brazil showed a single genotype (South America I) circulating in the country, with the exception of a single strain from Rondonia, which represented South America genotype II. Brazilian YFV strains have diverged into two clades; an older clade appears to have become extinct and another has become the dominant lineage in recent years. Pairwise nucleotide diversity between strains ranged from 0% to 7.4%, while amino acid divergence ranged from 0% to 4.6%. Phylogenetic analysis indicated traffic of virus variants through large geographic areas and suggested that migration of infected people may be an important mechanism of virus dispersal. Isolation of vaccine virus from a patient with a fatal case suggests that vaccine-related illness may have been misdiagnosed in the past.
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