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Updated: Aug 19, 2026
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
Published on: September 2, 2010
[Expression of pRb and E2F-1 and telomerase activity in ameloblastoma]
Ming Zhong1, Jie Wang, Bo Zhang
1Department of Pathology, College of Stomatology, China Medical University, Shenyang 110002, China. zhongming1234@tom.com
Objective:
To investigate the expression of pRb and E2F-1, and the association between their expression and the activity of telomerase (hTERT) or cyclin E in human ameloblastoma (AB), and to explore the clinical biological characteristics of AB.
Methods:
The expressions of pRb, E2F-1, cyclin E and hTERT mRNA in human AB were detected by in situ hybridization or immunohistochemistry (SP method).
Results:
The positive expression ratio of pRb in the cell nucleus of AB was 20.4% (11/54). The positive ratio of E2F-1, cyclin E and hTERT mRNA was 92.6% (50/54), 66.7% (36/54) and 94.4% (51/54), respectively. With AB recurrence and malignant transformation, the expression of hTERT, E2F-1, cyclin E was up-regulated. hTERT and cyclin E or E2F-1 mRNA had high positive relation (Spearsman'r(s) = 1.000, P = 0.0001).
Conclusions:
The regulatory pathway of Rb/E2F-1 is associated with the cell proliferation and in differentiation of AB. The activity or release of telomerase may be related to the lower expression of Rb and higher expression of E2F-1, and is up-regulated in G(1) late phase by cyclin E.
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