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Related Experiment Videos

Testosterone measurements in early infancy.

C Tomlinson1, H Macintyre, C A Dorrian

  • 1Department of Child Health, Royal Hospital for Sick Children, Glasgow, Scotland, UK.

Archives of Disease in Childhood. Fetal and Neonatal Edition
|October 23, 2004
PubMed
Summary

Neonatal testosterone levels vary by measurement method. Direct chemiluminescent immunoassay (Bayer ADVIA Centaur) is reliable for infant testosterone testing, but labs need method-specific reference ranges.

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Area of Science:

  • Endocrinology
  • Neonatal Medicine
  • Clinical Chemistry

Background:

  • Accurate measurement of circulating testosterone in neonates is crucial for assessing endocrine function.
  • Traditional radioimmunoassay (RIA) methods often involve solvent extraction, which can be time-consuming.
  • Direct immunoassays offer a potentially faster alternative but require validation for specific populations.

Purpose of the Study:

  • To compare testosterone concentrations in neonates measured by a direct chemiluminescent immunoassay (Bayer ADVIA Centaur) versus a traditional RIA method.
  • To determine the reliability of the Bayer ADVIA Centaur assay for neonatal testosterone measurement.
  • To establish method-specific reference ranges for neonatal testosterone levels.

Main Methods:

  • Direct comparison of testosterone measurements in infant serum samples using a Bayer ADVIA Centaur immunoassay and a solvent-extraction RIA.

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  • Analysis of samples from male and female infants across different age groups (<10 days and 10-50 days).

    • Main Results:

    • Neonatal circulating testosterone concentrations are significantly dependent on the assay method used.
    • The Bayer ADVIA Centaur assay demonstrated reliable performance in neonates.
    • Established reference ranges for male infants <10 days: 2.5-11.1 nmol/l; female infants <10 days: 1.7-5.6 nmol/l.
    • Established reference ranges for male neonates 10-50 days: 0.2-17.2 nmol/l; female neonates 10-50 days: 0.1-1.5 nmol/l.

    Conclusions:

    • The choice of assay method impacts neonatal testosterone measurements.
    • The Bayer ADVIA Centaur assay is a reliable tool for quantifying testosterone in neonates.
    • Laboratories should establish their own reference ranges when utilizing direct commercial immunoassays for neonatal testosterone testing.