Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Colonisation of Pathogens01:25

Colonisation of Pathogens

Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wound, Pressure Ulcer, and Burn Guidelines (2023)-6: Guidelines for the Management of Burns, Third Edition.

The Journal of dermatology·2026
Same author

Complete genome sequence of <i>Clostridium botulinum</i> TMIPH-CB191 (GTC 18335) isolated from a human in Japan.

Microbiology resource announcements·2026
Same author

Complete genome sequence of <i>Elizabethkingia</i> sp<i>.</i> GTC18459 isolated from a snake skin in Japan.

Microbiology resource announcements·2026
Same author

Electrochemical Tryptophan-Selective Bioconjugation in Neutral Buffer via Cooperative <i>N</i>-Oxyl Radicals.

Bioconjugate chemistry·2026
Same author

Complete genome sequence of <i>Francisella salimarina</i> GTC 22824 isolated from coastal seawater in Osaka, Japan.

Microbiology resource announcements·2026
Same author

Complete genome sequences of <i>Lichenicola</i> spp. GTC18330 and 18331 isolated from bee products in Gifu, Japan.

Microbiology resource announcements·2026

Related Experiment Video

Updated: Jul 7, 2026

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
11:04

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Published on: February 19, 2013

Lipopolysaccharide transport system across colonic epithelial cells in normal and infective rat.

Mikio Tomita1, Rie Ohkubo, Masahiro Hayashi

  • 1Department of Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan. tomita@ps.toyaku.ac.jp

Drug Metabolism and Pharmacokinetics
|October 23, 2004
PubMed
Summary

Colonic epithelial cells show specific transport systems for lipopolysaccharide (LPS), involving CD14 and Toll-like receptor 4 (TLR4). This transport is directional and temperature-dependent, suggesting active mechanisms in the rat intestine.

More Related Videos

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium
04:05

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium

Published on: May 31, 2024

Related Experiment Videos

Last Updated: Jul 7, 2026

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
11:04

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Published on: February 19, 2013

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium
04:05

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium

Published on: May 31, 2024

Area of Science:

  • Gastroenterology
  • Immunology
  • Cell Biology

Background:

  • Lipopolysaccharide (LPS) is a key component of Gram-negative bacteria.
  • Understanding LPS transport in the intestine is crucial for inflammatory bowel disease research.
  • CD14 and Toll-like receptor 4 (TLR4) are known receptors for LPS.

Purpose of the Study:

  • To investigate the transport mechanisms of LPS across rat colonic epithelial cells.
  • To examine the role of CD14 and TLR4 in LPS transport.
  • To determine if LPS exposure alters its own transport and receptor expression.

Main Methods:

  • FITC-labeled LPS (FITC-LPS) transport assays across colonic epithelial cells in diffusion chambers.
  • Examination of CD14 and TLR4 expression in normal and LPS-exposed rats.
  • Experiments conducted at different temperatures (4°C and 37°C) and with inhibitors (unlabeled LPS, anti-CD14, anti-TLR4 antibodies).

Main Results:

  • FITC-LPS demonstrated directional transport (mucosal to serosal) across colonic epithelium, significantly higher than the reverse direction.
  • Transport was temperature-dependent (37°C > 4°C) and inhibited by unlabeled LPS, anti-CD14, and anti-TLR4 antibodies in normal rats.
  • LPS exposure altered inhibition patterns and reduced CD14 expression on the mucosal side, while TLR4 inhibition remained effective for M to S transport.

Conclusions:

  • Rat colonic epithelial cells possess specific, directional transport systems for LPS.
  • CD14 and TLR4 are involved in LPS transport, with CD14's role potentially altered by prior LPS exposure.
  • These findings suggest active, receptor-mediated transport of LPS across the intestinal barrier.