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Related Experiment Videos

Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat.

Ju-Kyung Yu1, Trevor M Dake, Sukhwinder Singh

  • 1Department of Plant Breeding, Cornell University, 413 Bradfield Hall, Ithaca, NY 14850, USA.

Genome
|October 23, 2004
PubMed
Summary

This study developed new molecular markers from expressed sequence tags (ESTs) to improve wheat

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Area of Science:

  • Plant genetics and genomics
  • Molecular biology and bioinformatics

Background:

  • Expressed sequence tags (ESTs) are crucial for developing molecular markers.
  • Enhancing genetic maps and identifying functional gene loci in hexaploid wheat (Triticum aestivum L.) is essential for crop improvement.

Purpose of the Study:

  • To develop and map novel EST-derived simple sequence repeat (SSR) markers in wheat.
  • To enhance the resolution of the existing wheat genetic map and identify potential functional genes.

Main Methods:

  • Analysis of over 260,000 ESTs from five grass species to identify SSR-containing sequences.
  • Development and cross-species testing of primer pairs derived from EST-SSR sequences.
  • Integration of mapped EST-SSR loci into a reference wheat genetic map and chromosomal localization using nullisomic-tetrasomic lines.

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Main Results:

  • Identified 5418 SSR-containing sequences, developing 156 cross-species superclusters and 138 singletons.
  • 227 primer pairs amplified DNA from two or more species, with dinucleotide EST-SSRs showing higher polymorphism (74%) than trinucleotides (56%).
  • 90 EST-SSR markers mapped 149 loci on 19 wheat chromosomes, with 22 ESTs having putative functions identified. 80 markers localized 104 loci.

Conclusions:

  • Developed a set of valuable EST-SSR markers for wheat genetics.
  • The enhanced genetic map facilitates comparative mapping and the identification of genes influencing important wheat traits.