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Updated: Aug 21, 2026

Experimental Metastasis Assay
Published on: August 24, 2010
Genes upregulated in a metastasizing human colon carcinoma cell line
Véronique Orian-Rousseau1, Sigrun Mink, Jörg Mengwasser
1Forschungszentrum Karlsruhe, Institute of Toxicologie and Genetics, Karlsruhe, Germany.
Abstract:
Differential gene expression between the metastatic human colon cancer cell line HT29p and its nonmetastatic counterpart HT29-MTX was revealed by suppression subtractive hybridization. Fifty-eight individual genes showed increased mRNA levels in HT29p cells. Only 15 of these genes had been related to transformation in previous studies; the majority of genes are new candidates encoding proteins relevant for the metastatic process. Cancer profiling arrays as well as in situ hybridization study revealed that at least some of the genes obtained in the SSH screen are also differentially expressed in human tumors.
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