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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
Cecília Alves-Araújo1, Maria Judite Almeida, Maria João Sousa
1Center of Biology/Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Abstract:
The freeze stress responses to prolonged storage at -20 degrees C in Torulaspora delbrueckii PYCC5323 were investigated. In this yeast, no loss of cell viability was observed for at least 120 days during freezing at -20 degrees C, whereas a loss of 80% was observed in a commercial baker's yeast after 15 days. In the former strain, freeze resistance was dependent on an adaptation process. The primary cell target of freeze stress was the plasma membrane, preservation of its integrity being related with a lower increase of lipid peroxidation and with a higher resistance to H(2)O(2), but not with the intracellular trehalose concentration.
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