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Updated: Aug 21, 2026

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
Homology model of the multidrug transporter LmrA from Lactococcus lactis
Karin Pleban1, Antonio Macchiarulo, Gabriele Costantino
1Department of Pharmaceutical Chemistry, University of Vienna, Althanstrasse 14, A-1090 Wien, Austria.
Abstract:
LmrA is an ATP dependent multidrug transporter from Lactococcus lactis conferring antibiotic resistance to 17 out of 21 most frequently administered antibiotics. Starting from the dimeric crystal structure of Vc-MsbA, we built two homology models, with NBD:NBD interfaces reflecting the nonenergized and energized state, respectively. The TMD:TMD topology of the dimer is consistent with the previously obtained substrate photoaffinity labeling pattern suggesting binding of substrates at the TMD:TMD interface involving helix 3 of one monomer and helices 5 and 6 of the other monomer.
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