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Published on: September 1, 2015
[Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells]
Qing Shen1, Yu-jia Yao, Ru-ying Li
1Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mailsq@fmmu.edu.cn
Objective:
To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G(1)/S-phase renal tubule epithelial cells (RTECs).
Methods:
G1-phase RTECs were divided into cantharidin-treated group, exposed to sodium cyanide (CN) and cantharidin, hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography.
Results:
The concentration of CN exposure for 1 h, ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50+/-0.26 mmol/g protein, 4.25+/-0.11 mmol/g protein, 8.58+/-0.13 mmol/g protein, respectively, P<0.01).
Conclusion:
Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.
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