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Detection of antigen-antibody reaction using a fluorescent probe and its application to homogeneous competitive-type

Hiroshi Oneda1, Kuniyo Inouye

  • 1Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sankyo-ku, Kyoto 606-8502, Japan.

Bioscience, Biotechnology, and Biochemistry
|October 27, 2004
PubMed

The interaction of bovine insulin with anti-human insulin antibody (mAb) was examined using a fluorescent probe. The fluorescence intensity of fluoresceinthiocarbamyl (FTC)-insulin was increased by adding mAb, and the increase was saturated at 53% at a molar ratio of FTC-insulin to mAb of 2.0. Based on the change in fluorescence intensity, a standard curve of the homogeneous competitive-type immunoassay was constructed, and the detection range of insulin was found to be 50-400 nM.

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