Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Initial salivary pellicle formation on solid substrates studied by AFM.

M Hannig1, A Döbert, R Stigler

  • 1Department of Operative Dentistry and Periodontology, Saarland University, Building 73, D-66421 Homburg/Saar, Germany.

Journal of Nanoscience and Nanotechnology
|October 27, 2004
PubMed
Summary

Salivary pellicle, organic layers on oral tissues, impacts bacterial adherence. This study used atomic force microscopy (AFM) to reveal the initial pellicle

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Equine dental destructive disorders: an epidemiological survey in northern Germany.

Frontiers in veterinary science·2026
Same author

Snapshot of the <i>Seconds</i>-Pellicle: Ultrastructure and Proteomic Changes.

Journal of dental research·2025
Same author

Morphological characterization of 3D cell cultures generated by liquid overlay technique.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2025
Same author

Bovine milk and milk protein- promotor or inhibitor of bacterial biofilm formation at the tooth surface?

BMC oral health·2025
Same author

Impact of pH-adjusted fluoride and stannous solutions on the protective properties on the pellicle layer in vitro and in situ.

Scientific reports·2024
Same author

Erosion behaviour of human, bovine and equine dental hard tissues.

Scientific reports·2023

Area of Science:

  • Oral biology
  • Biomaterials science
  • Surface chemistry

Background:

  • Salivary pellicle is crucial for oral surface phenomena.
  • Its initial formation influences bacterial adhesion.
  • Detailed understanding of pellicle ultrastructure is lacking.

Purpose of the Study:

  • To investigate the initial salivary pellicle formation in situ.
  • To elucidate the morphogenesis and ultrastructure of the pellicle.
  • To explore the role of substrate surface energy.

Main Methods:

  • Atomic Force Microscopy (AFM) in tapping mode.
  • In situ analysis of pellicle formation on mica, silicon wafer, and graphite.
  • Characterization of adsorbed salivary biopolymer layer.

Related Experiment Videos

Main Results:

  • Salivary pellicles exhibit a globular surface morphology.
  • Average diameter of adsorbed salivary proteins: 15 ± 3 nm.
  • Average height of adsorbed salivary proteins: 2.0 ± 0.5 nm.
  • Substrate surface energy affects pellicle formation rate.
  • Adsorbed protein size is consistent across different substrates.

Conclusions:

  • AFM provides novel insights into initial pellicle morphogenesis.
  • Pellicle formation is influenced by substrate properties.
  • Understanding pellicle structure is key to controlling oral surface interactions.