Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ocular tissue imaging using ultrahigh-resolution, full-field optical coherence tomography.

Kate Grieve1, Michel Paques, Arnaud Dubois

  • 1Laboratoire d'Optique Physique, Ecole Supérieure de Physique et Chimie Industrielles, Centre National de la Recherche Scientifique, Paris, France. grieve@optique.espci.fr

Investigative Ophthalmology & Visual Science
|October 27, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

En face localization of retinal telangiectatic capillaries using OCT compared with ICG angiography in chronic vascular macular oedema.

Acta ophthalmologica·2026
Same author

Preclinical Safety and Feasibility Study of Line-Field Confocal Optical Coherence Tomography for Ophthalmology Applications.

Translational vision science & technology·2026
Same author

Magnetoencephalography biomarkers for assessing myelin content and neuronal function in acute optic neuritis.

Brain communications·2026
Same author

The ITM2B-associated retinal dystrophy mutation modifies BRI23 peptide interactions in the human retina.

Scientific reports·2026
Same author

Needle-Shaped Retinal Deposits and Bilateral Neovascular Glaucoma Revealing Transthyretin Amyloidosis.

Ophthalmology. Retina·2026
Same author

Bidirectional Retro mode differential imaging improves drusen boundary depiction in early and intermediate AMD: a pilot study.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026

Full-field optical coherence tomography (OCT) achieves cellular-level imaging of unstained ocular tissues. This noninvasive technique shows promise for future in vivo eye examinations.

Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Optical Engineering

Background:

  • Optical Coherence Tomography (OCT) is a noninvasive imaging modality.
  • Full-field OCT (FFOCT) offers rapid, scanning-free tomographic imaging.
  • Advancements in FFOCT aim to improve resolution and penetration depth for ocular applications.

Purpose of the Study:

  • To evaluate the capabilities of ultrahigh-resolution, full-field OCT for ocular tissue imaging.
  • To explore the potential of FFOCT for noninvasive, cellular-level visualization of eye structures.

Main Methods:

  • A Linnik-type interferometer with a tungsten-halogen source was utilized for FFOCT.
  • The system achieved a spatial resolution of 0.9 x 0.7 microm (transverse x axial).
  • Unstained ocular tissues and whole eyes from rodents and pigs were imaged, with real-time en face tomographic images acquired in approximately 1 second.

Related Experiment Videos

Main Results:

  • Cellular-level resolution was demonstrated in various ocular tissues, including cornea, lens, and retina.
  • En face and reconstructed images clearly delineated cellular structures and microvasculature.
  • Transscleral imaging of the retina was successful in albino animals.

Conclusions:

  • Ultrahigh-resolution FFOCT provides cellular detail in unstained ocular tissues with significant penetration.
  • The technique's simplicity and potential for in vivo imaging are highlighted.
  • Further development is underway to adapt this technology for clinical use.