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Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
Elevated pro-inflammatory CD4+CD28- lymphocytes and stroke recurrence and death.
Z G Nadareishvili1, H Li, V Wright
1Stroke Branch, National Institute of Neurologic Disorders and Stroke, NIH, 10 Center Dr., Bethesda, MD 20892-1294, USA.
Neurology
|October 27, 2004
Summary
Elevated counts of CD4+CD28- T-cells in the blood indicate a higher risk of recurrent stroke or death after an initial ischemic stroke. This finding suggests a potential biomarker for stroke outcomes.
Area of Science:
- Immunology
- Neurology
- Cardiovascular Medicine
Background:
- The CD4+CD28- T-cell subset possesses pro-inflammatory and tissue-damaging properties.
- Understanding the role of specific immune cell subsets in post-stroke complications is crucial for risk stratification.
Purpose of the Study:
- To investigate the association between the expansion of CD4+CD28- T-cells and the risk of recurrent stroke or death following acute ischemic stroke.
Main Methods:
- A prospective study of 106 patients admitted within 48 hours of ischemic stroke.
- Quantification of peripheral blood CD4+CD28- cells using flow cytometry.
- Follow-up for 1 year to record recurrent stroke or death.
Main Results:
- Higher CD4+CD28- T-cell counts were significantly associated with increased rates of recurrent stroke and death.
- Patients with CD4+CD28- counts >8.0% had a 5.81-fold increased hazard ratio for stroke recurrence or death, even after adjusting for key risk factors.
- Elevated CD4+CD28- counts were also linked to a history of prior stroke.
Conclusions:
- Increased circulating CD4+CD28- T-cells serve as a biomarker for elevated risk of recurrent stroke and mortality.
- The expansion of this T-cell subset may play a pathogenic role in the progression of cerebrovascular disease.
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