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The human serum amyloid A locus SAA4 is a pseudogene
1Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, MD.
Biochemical and Biophysical Research Communications
|March 26, 1992
Summary
Researchers isolated the human genomic DNA clone GSAA4, identifying it as a pseudogene within the serum amyloid A (SAA) gene family. This discovery completes the set of clones necessary for comprehensive human SAA gene analysis.
Area of Science:
- Genomics
- Molecular Biology
- Human Genetics
Background:
- The human serum amyloid A (SAA) gene family plays a role in inflammatory responses.
- Previous studies have identified several SAA genes, but a complete collection of genomic clones was lacking.
Purpose of the Study:
- To isolate and characterize the human genomic DNA clone GSAA4.
- To complete the set of clones representing the human SAA gene family.
- To identify potential pseudogenes within the SAA locus.
Main Methods:
- Isolation of the GSAA4 clone from a size-selected Bgl II library using hybridization.
- Sequencing of the 5' end of the GSAA4 clone.
- Restriction mapping of the GSAA4 clone.
Main Results:
- The GSAA4 clone was successfully isolated and sequenced.
- Sequence analysis revealed similarity to the first exon of the SAA1 gene, but with significant differences and a mutated splice site, indicating a pseudogene.
- Restriction mapping confirmed GSAA4 corresponds to the SAA4 locus.
- The isolation of GSAA4 completes the genomic clone collection for human SAA genes.
Conclusions:
- The GSAA4 clone represents a pseudogene within the human serum amyloid A gene family.
- This work provides a complete set of genomic clones for the human SAA locus, facilitating further research.
- The findings contribute to a comprehensive understanding of the human SAA gene family's structure and evolution.