Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental changes in intestinal glucose transporter mRNA levels.

K Miyamoto1, K Hase, Y Taketani

  • 1Department of Nutrition, School of Medicine, The University of Tokushima, Japan.

Biochemical and Biophysical Research Communications
|March 16, 1992
PubMed
Summary

This study tracked changes in intestinal glucose transporter mRNA in developing rats. Key transporters like GLUT5 and GLUT2 showed significant developmental shifts in the third week after birth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to 'A phase II trial of paclitaxel plus biweekly cetuximab for patients with recurrent or metastatic head and neck cancer previously treated with both platinum-based chemotherapy and anti-PD-1 antibody': [ESMO Open volume 9 issue 6 (2024) 103476].

ESMO open·2024
Same author

A phase II trial of paclitaxel plus biweekly cetuximab for patients with recurrent or metastatic head and neck cancer previously treated with both platinum-based chemotherapy and anti-PD-1 antibody.

ESMO open·2024
Same author

Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy.

Autophagy·2022
Same author

Open-label phase II study of the efficacy of nivolumab for cancer of unknown primary.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Prognostic and predictive values of tumour budding in stage IV colorectal cancer.

BJS open·2020
Same author

Effect of tumor burden and growth rate on treatment outcomes of nivolumab in head and neck cancer.

International journal of clinical oncology·2020

Area of Science:

  • Physiology
  • Molecular Biology
  • Developmental Biology

Background:

  • Glucose absorption in the jejunum is crucial for infant nutrition.
  • Specific glucose transporters play vital roles in nutrient uptake.
  • Understanding developmental regulation of these transporters is essential.

Purpose of the Study:

  • To investigate developmental changes in mRNA levels of key glucose transporters (SGLT1, GLUT5, GLUT2) in the rat jejunum.
  • To identify the specific postnatal periods of significant transporter gene expression changes.

Main Methods:

  • Slot blot RNA analysis was employed to quantify mRNA levels.
  • Samples were collected from rats at various ages, from 5 days to adulthood.
  • Levels of SGLT1, GLUT5, and GLUT2 mRNA were measured.

Related Experiment Videos

Main Results:

  • SGLT1 mRNA levels remained relatively constant throughout development.
  • GLUT5 mRNA peaked at 10 days and declined to adult levels by day 20.
  • GLUT2 mRNA was low initially, increasing significantly to reach adult levels by day 25.

Conclusions:

  • Intestinal glucose transporter gene expression undergoes substantial changes during the third week after birth.
  • GLUT5 and GLUT2 exhibit distinct developmental regulation patterns.
  • These findings highlight critical developmental shifts in intestinal glucose transport mechanisms.