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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
Published on: December 8, 2010
Immunodetection of UCP1 in rat thymocytes
A M Carroll1, L R Haines, T W Pearson
1Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland.
Biochemical Society Transactions
|October 28, 2004
Abstract:
Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This implies that rat thymocytes contain uncoupling protein 1.
