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p62 is involved in the mechanism of Mallory body formation

Li Nan1, Yong Wu, Fawzia Bardag-Gorce

  • 1Department of Pathology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Experimental and Molecular Pathology
|October 28, 2004
PubMed

Insights

p62 protein is crucial for Mallory body formation in liver cells. Inhibiting p62 significantly reduced Mallory body development, while overexpressing it accelerated their formation.

Area of Science:

  • Hepatology
  • Cell Biology
  • Protein Degradation

Background:

  • p62 is a scaffolding protein that binds polyubiquitin and participates in proteasomal degradation.
  • Mallory bodies (MBs) are protein aggregates found in hepatocytes, implicated in liver injury.

Purpose of the Study:

  • To investigate the role of p62 in the formation of Mallory bodies (MBs).

Main Methods:

  • Primary mouse hepatocytes were cultured and treated with drug-priming.
  • p62 expression was inhibited using gene-specific gripNA (gp62).
  • p62 overexpression was achieved via transfection with p62-green fluorescent protein (GFP) plasmid.

Main Results:

  • gp62 treatment inhibited MB formation by 94% in drug-primed hepatocytes, decreasing levels of p62, ubiquitin (Ub), and cytokeratin 8 (CK8).
  • Overexpression of p62 enhanced MB formation by 339% in drug-primed hepatocytes.
  • p62 overexpression in normal hepatocytes induced Ub-positive, CK8-negative MB-like aggresomes.

Conclusions:

  • p62 plays a critical role in the mechanism of Mallory body formation.
  • Modulating p62 levels affects the development of hepatic protein aggregates.

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