Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammatory response and the endothelium.

P L Meroni1, M O Borghi, E Raschi

  • 1Department of Internal Medicine, University of Milan, Istituto Auxologico Italiano, Milan, Italy. pierluigi.meroni@unimi.it

Thrombosis Research
|October 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pregnancy in patients affected by axial-spondyloarthritis: a narrative review of disease activity and obstetric outcomes.

Reumatismo·2024
Same author

The history of antiphospholipid syndrome.

Reumatismo·2023
Same author

Transplantation on a Posidonia oceanica meadow to facilitate its recovery after the Concordia shipwrecking.

Marine pollution bulletin·2022
Same author

Metal removal processes in a pilot hybrid constructed wetland for the treatment of semi-synthetic stormwater.

The Science of the total environment·2020
Same author

Reteporella spp. success in the re-colonization of bare coralligenous reefs impacted by Costa Concordia shipwreck: The pioneer species you did not expect.

Marine pollution bulletin·2020
Same author

Secukinumab for patients with plaque psoriasis affected by multiple sclerosis: a mini-review with a representative case report.

Journal of the European Academy of Dermatology and Venereology : JEADV·2019

Antiphospholipid antibodies may not cause significant endothelial dysfunction alone in antiphospholipid syndrome (APS). Further research is needed to understand the in vivo mechanisms of APS-associated vasculopathy.

Area of Science:

  • Immunology
  • Vascular Biology
  • Rheumatology

Background:

  • Antiphospholipid antibodies (aPL) are implicated in antiphospholipid syndrome (APS)-associated vasculopathy.
  • In vitro studies show aPL activate endothelial cells (EC), inducing pro-inflammatory and pro-coagulant phenotypes.
  • Evidence for in vivo endothelial perturbation by aPL remains limited.

Purpose of the Study:

  • To investigate in vivo endothelial dysfunction in patients with antiphospholipid syndrome (APS).
  • To assess indirect ex vivo parameters of endothelial dysfunction and circulating endothelial cells.

Main Methods:

  • Evaluated plasma levels of soluble adhesion molecules (sADM), soluble thrombomodulin (sTM), von Willebrand factor (vWF), and tissue plasminogen activator (t-PA).
  • Assessed circulating endothelial cells (CEC) via flow cytometry and brachial artery flow-mediated vasodilation (FMV).

Related Experiment Videos

  • Included patients with primary APS, secondary APS (SLE), and asymptomatic antiphospholipid positivity, compared to healthy controls.

    • Main Results:

    • No significant difference in sADM levels between patients and controls.
    • A significant increase in von Willebrand factor (vWF) titres was observed in patients (P<0.05).
    • No significant differences in CEC counts or FMV were found.

    Conclusions:

    • Antiphospholipid antibodies alone may not induce significant endothelial perturbation in vivo.
    • Findings suggest a potential 'two-hit' hypothesis for APS-associated vasculopathy, similar to animal models.