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Updated: Aug 21, 2026
Viral Tracing of Genetically Defined Neural Circuitry
Published on: October 17, 2012
Culturing primary and transformed neuronal cells for studying pseudorabies virus infection
Toh Hean Ch'ng1, E Alexander Flood, Lynn William Enquist
1Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Abstract:
This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.
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