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Malaria drug-susceptibility testing. HRP2-based assays: current data, future perspectives.

Harald Noedl1, Walther H Wernsdorfer, Herwig Kollaritsch

  • 1Department of Specific Prophylaxis and Tropical Medicine, Institute of Pathophysiology, University of Vienna, Vienna, Austria. harald.noedl@univie.ac.at

Wiener Klinische Wochenschrift
|October 29, 2004
PubMed
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A new histidine-rich protein II (HRP2) assay offers a simpler, faster method for malaria drug susceptibility testing. This HRP2 assay shows high sensitivity and accuracy, potentially replacing traditional, complex in-vitro methods.

Area of Science:

  • Medical Parasitology
  • Biotechnology
  • Drug Discovery

Background:

  • In-vitro drug-susceptibility assays are crucial for malaria drug development and resistance surveillance.
  • Traditional assays are often time-consuming, require specialized expertise, and extensive laboratory infrastructure.

Purpose of the Study:

  • To develop a novel, simplified drug sensitivity assay for Plasmodium falciparum.
  • To establish an assay based on the measurement of histidine-rich protein II (HRP2).

Main Methods:

  • Developed a double-site sandwich ELISA using HRP2 to quantify Plasmodium falciparum growth and drug inhibition.
  • Compared HRP2 assay results with isotope assays and WHO schizont maturation tests.

Main Results:

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  • The HRP2 assay demonstrated results closely paralleling traditional methods (P < 0.001).
  • The assay is rapid (2-3 hours) and requires minimal technical equipment.
  • Preliminary field studies indicated similar outcomes to the WHO assay (R = 0.942).

Conclusions:

  • The HRP2 assay is a sensitive, simple, and rapid tool for malaria drug sensitivity testing.
  • This assay has potential applications in epidemiological studies and new drug screening.
  • The HRP2 assay may replace traditional in-vitro drug-sensitivity techniques due to its advantages.