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Updated: Aug 21, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles
Valéria Abrantes Pinheiro Carvalho1, Darcy de Oliveira Tosello, Miguel Angel de Castillo Salgado
1Department of Biosciences and Oral Diagnosis, São José dos Campos School of Dentistry, São Paulo State University (UNESP), São Jose dos Campos, São Paulo, Brazil. valcarvalho@directnet.com.br
Purpose:
The aim of this work was to evaluate the effectiveness of homogenous demineralized dentin matrix (HDDM) slices in surgical bone defects created in the mandibles of rabbits and occluded with a polytetrafluoroethylene (PTFE) membrane in the promotion of bone growth.
Materials And Methods:
Surgical bone defects were created in 36 adult rabbits and divided into 4 groups: bone defect (control), bone defect with PTFE membrane, bone defect with HDDM, and bone defect with both HDDM and a PTFE membrane (HDDM + PTFE). The rabbits were sacrificed after 30, 60, and 90 days, and the bone defects were examined histologically and by histomorphometric analysis (analysis of variance and the Tukey test).
Results:
The volume of newly formed bone matrix was significantly greater in the HDDM and HDDM + PTFE groups than in the control and PTFE groups. The discrete inflammatory reaction found in the HDDM and HDDM + PTFE groups did not prevent the osteopromotive activity of the dentin matrix.
Discussion:
HDDM slices were biocompatible and were resorbed during the bone remodeling process. They stimulated the newly formed bone until 30 days after implantation.
Conclusion:
Bone repair was accelerated in the bone defects treated with HDDM in comparison to the control group.
