Human cell culture studies with dental metallic materials

The compatibility of cast non-alloyed titanium and eight dental castable alloys (precious, low noble, non-precious) was determined by means of human primary oral fibroblasts derived from the attached gingiva and the periodontium. Furthermore, cast non-alloyed Ti was tested in a human permanent cell culture (MOLT 4). All investigated metallic materials showed cytotoxic properties in primary cultures. The differences in cytotoxicity between the various materials were statistically significant. The best results were found for one noble alloy and for non-alloyed titanium, whereas all non-precious alloys almost completely inhibited cell growth. The cytotoxic potential of the investigated materials might contribute to the irritating effect of dental cast restorations adjacent to the gingiva and the periodontium.