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Miniature tissue microarrays for HercepTest standardisation and analysis
C Gulmann1, P Loring, A O'Grady
1Department of Pathology, Beaumont Hospital and Royal College of Surgeons in Ireland, Dublin 9, Ireland. cgulmann@rcsi.ie
Journal of Clinical Pathology
|October 29, 2004
Summary
A miniature tissue microarray (TMA) containing various HercepTest scores serves as an effective control for immunohistochemistry. This reliable tissue control slide can be stored for up to 270 days.
Area of Science:
- Oncology
- Pathology
- Biotechnology
Background:
- Immunohistochemistry (IHC) is crucial for cancer diagnosis and treatment selection.
- Accurate scoring of IHC assays, such as HercepTest, is essential for patient management.
- Standardized controls are needed to ensure the reliability of IHC staining results.
Purpose of the Study:
- To evaluate the feasibility of using a miniature tissue microarray (TMA) as a control for HercepTest immunohistochemistry.
- To determine the utility of a TMA containing diverse HercepTest scores (0 to 3+) for routine IHC.
Main Methods:
- A 5x5 mm TMA was created using breast cancer cases with known HercepTest scores (0, 1+, 2+, 3+).
- The TMA included five 0.6 mm cores per score from 20 distinct cases, constructed in under an hour.
- Multiple 4-micrometer sections were cut and stored at 4°C for up to 270 days.
Main Results:
- All prepared slides exhibited intact tissue spots.
- Immunohistochemical staining on the TMA remained consistent throughout the storage period.
Conclusions:
- A miniature TMA with representative HercepTest scores is an ideal tissue control for IHC.
- This TMA serves as a valuable visual reference for accurate case scoring.
- Stored slides maintained their integrity and utility for up to 270 days.