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Updated: Aug 21, 2026

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency
Published on: November 10, 2021
Influence of amphotericin B on the ciliary beat frequency of nasal mucosa
Edmund Hofer1, Andreas Neher, Anneliese Schrott-Fischer
1Department of Otorhinolaryngology, University Hospital of Innsbruck, Innsbruck, Austria.
Objective:
A new therapeutic approach in eosinophilic fungal rhinosinusitis is topical application of antifungals such as amphotericin B. The tolerability of a recently applied concentration of this drug by human nasal mucosa was tested in vitro.
Methods:
Ciliary beat frequency (CBF) was measured by a photometric technique, combining a light microscope, a photometer, a photograph-multiplier, and a computed analyzing unit.
Results:
Treatment for 20 minutes with amphotericin B finally diluted in saline to 0.1 mg/mL caused no decrease in CBF. By contrast, both amphotericin B diluted in distilled water and distilled water without additives lowered CBF about 50%. This decrease was not reversible after reincubation for 20 minute in saline.
Conclusions:
The results confirm the good tolerability of topical amphotericin B in the nasal and paranasal cavity. Because of the negative effect of distilled water, final dilution in physiologic solvents should be considered.
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