[Effects of advanced glycation end products and hypoxia on endothelin-1 secretion by bovine retinal microvascular pericytes]
Baihua Chen1, Deyong Jiang, Luosheng Tang
1Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
Purpose:
To investigate the effects of advanced glycation end products(AGE) and hypoxia on endothelin-1 (ET-1) secretion by cultured bovine retinal microvascular pericytes (BRPs).
Methods:
BRPs were incubated with different concentrations of AGE (8, 32, 125 microg/ ml) for four days, hypoxia (10% O2, 5% CO2, 85% N2) for 12, 24, 48 hours, respectively and AGE for 2 days firstly and then hypoxia for 48 hours. Finally, ET-1 production was assessed in culture supernatants of BRPs under basal and stimulated conditions by radioimmunoassay.
Results:
Hypoxia stimulated ET-1 production in a time-dependent manner in cultured BRPs. AGE could not induce the production significantly, but it could upregulate the effect of hypoxia on ET-1.
Conclusion:
These results suggested that AGE and hypoxia could regulate the ET-1 production in BRPs. ET-1 might be related to microvascular dysfunction in diabetes.
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