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Updated: Aug 21, 2026

Wholemount In Situ Hybridization for Astyanax Embryos
Published on: March 2, 2019
[Expression pattern of profilin homologue in embryogenesis of amphioxus]
Xiao Hui Zhang1, Hui Yang, Xiang Wei Huang
1Institute of Developmental Biology, Life Science College, Shandong University, Jinan 250100.
Abstract:
The expression pattern of profilin homologue in embryogenesis of amphioxus was characterized by whole-mount in situ hybridization combined serial histological sections and Northern blot. The results revealed that the dynamic expression pattern of the profilin homologue just accorded with muscle differentiating in this animal. It was most possibly involved in the development of muscle in amphioxus. The result of Southern blot supported that there was only one copy of profilin homologue in the genome of Qingdao amphioxus. It may be good for explaining the molecular mechanism of muscle differentiation in amphioxus.

