[Activity assay, antiserum preparation and cellular localization of ustiloxins]

False smut is an increasingly important rice disease in recent years, caused by a pathogen, Ustilaginoidea virens Cooke, Takahashi. The pathogen fungus grew well in PD medium, and could produce toxins, ustiloxins, which strongly inhibited the growth of radicle and embryo of plants. The rough ustiloxins were extracted with 100% methanol from the culture filtrate in the study. The antiserum against Ustiloxin A, the main component of the toxins, was raised by immunizing New Zealand rabbit. Elisa assay showed that the titers of the two antiserums obtained were 1: 20 000 and 1: 6 000, respectively. The specificity of the antiserums was also confirmed by Immuno-gold labeling.