Related Experiment Video
Updated: Aug 21, 2026

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder
Published on: June 23, 2023
[Ethanol affects the function of gamma-aminobutyric acid transporter I in mice brain]
Jie Jiang1, Jia Hua Hu, Min Wu
1College of Life Sciences, Zhejiang University, Hangzhou 310027.
Abstract:
It has been suggested that GABAergic system was involved in ethanol tolerance and addiction. Our previous work indicated both acute and chronic ethanol treatment enhanced the activity of GABA transporters in mouse brain. However, the expression level of GAT1 in CNS does not change, neither does the affinity of GABA transporters. In the present study, we demonstrated that GAT1 distribution on presynaptic membrane was altered after acute and chronic ethanol administration. Furthermore, the phosphorylation levels of GAT1 on three sites including Ser, Tyr and Thr were decreased. These data suggested that ethanol enhanced the function of GATs by means of inducing GAT1 redistribution in the cell and dephosphorylation may play a role in this process.
Related Concept Videos
Gut-Brain Axis
Hepatic Encephalopathy

