Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

C-terminal sequences direct cyclin D1-CRM1 binding.

Sharon Benzeno1, J Alan Diehl

  • 1Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, Department of Cancer Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.

The Journal of Biological Chemistry
|October 30, 2004
PubMed
Summary

A specific region in cyclin D1 controls its nuclear export, which is essential for cell cycle regulation. Disrupting this export mechanism can lead to uncontrolled cell growth and potentially cancer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mono-ADP-ribosylation-driven immunosuppression and cross-resistance to therapy through cancer cell intrinsic and extrinsic mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

A PML1-CCL5-PI3K/MAPK feedback loop governs survival of endocrine-resistant breast cancer cells.

Cell death and differentiation·2026
Same author

Stage-Specific Degradation of Cyclin D3 Orchestrates Myelopoiesis and Restrains Myeloproliferative Neoplasms Development.

Cancer research·2026
Same author

SPTBN2 promotes an immunosuppressive tumor microenvironment and cross-resistance to anti-cancer therapies.

bioRxiv : the preprint server for biology·2026
Same author

Editor's Note: Cell Cycle-Dependent and Schedule-Dependent Antitumor Effects of Sorafenib Combined with Radiation.

Cancer research·2026
Same author

Human antibody targeting of coronavirus spike S2 subunit is associated with protection mediated by Fc effector functions.

Journal of virology·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Cyclin D1 is a key regulator of the cell cycle, controlling progression through the G1 phase.
  • GSK-3beta phosphorylation at Thr-286 is known to promote cyclin D1 nuclear export via CRM1.
  • The precise mechanism and specific residues mediating CRM1 association remain incompletely understood.

Purpose of the Study:

  • To identify the specific amino acid residues in cyclin D1 responsible for mediating CRM1 association.
  • To investigate the functional consequences of disrupting cyclin D1 nuclear export on cell cycle regulation and cellular transformation.

Main Methods:

  • Site-directed mutagenesis to disrupt a hydrophobic patch (amino acids 290-295) in cyclin D1.
  • Analysis of cyclin D1 localization in murine fibroblasts using immunofluorescence.

Related Experiment Videos

  • Assessment of cyclin D1-CDK4 complex activity and their role in cellular transformation.

    • Main Results:

    • A hydrophobic patch (residues 290-295) adjacent to Thr-286 was identified as critical for CRM1-mediated nuclear export of cyclin D1.
    • Disruption of this hydrophobic patch resulted in constitutively nuclear cyclin D1-CDK4 complexes.
    • Constitutively nuclear cyclin D1-CDK4 complexes exhibited an enhanced propensity to potentiate the transformation of murine fibroblasts.

    Conclusions:

    • Deregulation of cyclin D1 nuclear export, mediated by the identified hydrophobic patch, is implicated in the aberrant cell cycle control observed in neoplastic growth.
    • Targeting the CRM1-dependent nuclear export pathway of cyclin D1 may offer potential therapeutic strategies for cancers driven by dysregulated cell cycle progression.