Related Experiment Video
Updated: Aug 21, 2026

Introductory Analysis and Validation of CUT&RUN Sequencing Data
Published on: December 13, 2024
BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison
Daniela Bartels1, Sebastian Kespohl, Stefan Albaum
1Universität Bielefeld, Center for Biotechnology (CeBiTec) D-33594 Bielefeld, Germany.
Summary:
We provide the graphical tool BACCardI for the construction of virtual clone maps from standard assembler output files or BLAST based sequence comparisons. This new tool has been applied to numerous genome projects to solve various problems including (a) validation of whole genome shotgun assemblies, (b) support for contig ordering in the finishing phase of a genome project, and (c) intergenome comparison between related strains when only one of the strains has been sequenced and a large insert library is available for the other. The BACCardI software can seamlessly interact with various sequence assembly packages.
Motivation:
Genomic assemblies generated from sequence information need to be validated by independent methods such as physical maps. The time-consuming task of building physical maps can be circumvented by virtual clone maps derived from read pair information of large insert libraries.
Related Concept Videos
Genome Annotation and Assembly
Genomics
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing
Applications of Molecular Taxonomy
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
