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Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.

Martin Beck¹, Friedrich Förster, Mary Ecke

¹Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.

Science (New York, N.Y.)

|October 30, 2004

Summary

Nuclear pore complexes (NPCs) act as cell gateways. Cryo-electron tomography revealed the central plug/transporter (CP/T) varies in position and volume, suggesting it represents cargo moving through the NPC scaffold.

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Area of Science:

Cell biology
Structural biology
Biophysics

Background:

Nuclear pore complexes (NPCs) regulate transport between the nucleus and cytoplasm.
Understanding NPC structure is crucial for deciphering nucleocytoplasmic exchange mechanisms.

Purpose of the Study:

To analyze the near-life structure of transport-active NPCs.
To investigate the dynamic structural states of NPCs during transport.

Main Methods:

Cryo-electron tomography of intact nuclei from Dictyostelium discoideum.
In silico subvolume extraction, 3D classification, and averaging of NPCs.

Main Results:

Distinct structural states of NPCs were identified.
The central plug/transporter (CP/T) exhibited variable volume and position.
CP/T positional changes correlated with rearrangements in the NPC scaffold.

Conclusions:

The CP/T's dynamic nature supports its role as cargo in transit.
NPC scaffold undergoes structural rearrangements associated with transport activity.