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Localization of proteins forming the outer surface of isolated metaphase chromosomes
N D Belyaev1, V G Budker, V A Dubrovskaya
1Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
FEBS Letters
|February 3, 1992
Abstract:
The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.