Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temporal changes in tPA and PAI-1 after maximal exercise.

Jamie A Cooper1, Paul R Nagelkirk, Adam M Coughlin

  • 1Human Energy Research Laboratory, Department of Kinesiology, Michigan State University, East Lansing, MI, USA.

Medicine and Science in Sports and Exercise
|October 30, 2004
PubMed
Summary

Accurate assessment of tissue plasminogen activator (t-PA) post-exercise requires blood draws within two minutes. Fibrinolytic markers like t-PA and PAI-1 change rapidly after maximal exercise.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of verification stage intensity and duration on [Formula: see text] determination.

European journal of applied physiology·2026
Same author

Dose-Response Effects of Pecan Consumption on Blood Lipid Profiles in Adults with Excess Body Weight and/or Dyslipidemia: a Randomized Controlled Trial.

The Journal of nutrition·2026
Same author

Sex Differences in Measures of Energy Expenditure and Body Composition in Young, Middle-Aged, and Older Adults.

Current developments in nutrition·2026
Same author

Running Performance in Response to Different Forms of Carbohydrate Through Cephalic-Phase Responses.

International journal of sports physiology and performance·2026
Same author

Comparison of blood lipid responses to high polyunsaturated fatty acid compared with high monounsaturated fatty acid dietary interventions: a systematic review and meta-analysis.

The American journal of clinical nutrition·2026
Same author

Dose-Response Effects of Cottonseed Oil on Blood Lipid Responses in Adults at Risk of Cardiovascular Disease: A Randomized Controlled Trial.

The Journal of nutrition·2025

Area of Science:

  • Exercise physiology
  • Hemostasis and thrombosis
  • Biochemistry

Background:

  • Fibrinolysis, the breakdown of blood clots, increases during acute exercise.
  • However, fibrinolytic activity declines rapidly in the post-exercise recovery period.
  • This rapid change necessitates careful consideration of blood sampling timing.

Purpose of the Study:

  • To investigate the time-dependent changes in tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) following acute maximal exercise.
  • To determine the optimal time window for blood sample collection to accurately reflect exercise-induced hemostatic responses.

Main Methods:

  • Eight healthy males underwent a graded maximal exercise test on a treadmill.
  • Venous blood samples were collected pre-exercise and at 1, 2, 4, 6, 8, and 10 minutes post-exercise.

Related Experiment Videos

  • Repeated measures ANOVA was used to analyze changes in t-PA activity, t-PA antigen, and PAI-1 activity.

    • Main Results:

    • Both t-PA activity and antigen levels significantly increased post-exercise.
    • t-PA levels remained elevated at 1 and 2 minutes but decreased significantly by 4 minutes post-exercise.
    • PAI-1 levels decreased from pre- to post-exercise but showed no significant changes within the 10-minute post-exercise period.

    Conclusions:

    • Blood sample collection for t-PA assessment after acute exercise should occur within 2 minutes of cessation.
    • This timing is crucial for accurately capturing the transient increase in t-PA activity and antigen levels.
    • PAI-1 levels appear less sensitive to immediate post-exercise timing within this window.