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Updated: Aug 3, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
Intein-mediated purification of a recombinantly expressed peptide
John A Pezza1, Karen N Allen, Dean R Tolan
1Department of Biology, Boston University, 5 Cummington Street, Boston, MA, USA.
Abstract:
A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.
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