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Solvent-dependent aggregation of a water-soluble poly(fluorene) controls energy transfer to chromophore-labeled DNA

Shu Wang1, Guillermo C Bazan

  • 1Department of Material and Chemistry, Institute for Polymers and Organic Solids, University of California, Santa Barbara, CA 93106, USA.

Chemical Communications (Cambridge, England)
|October 30, 2004
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...