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Detection of anti-HLA-F antibodies in sera from cancer patients
Katsuo Noguchi1, Manami Isogai, Eri Kuwada
1Institute of Molecular Immunology, MEDINET Co., Ltd, 2-2-8 Tamagawadai, Setagaya-ku, Tokyo, Japan. noguchi@medinet-inc.co.jp
Background:
We previously reported that a Q5 gene product (Q5 antigen) on the surface of tumor cells caused humoral immune reaction in syngeneic tumor-bearing mice. Transcripts of the Q5 gene were observed in various tumor cells. The human counterpart of the Q5 gene has not been clarified yet.
Materials And Methods:
Transcripts of human class Ib genes in various human tumor cell lines were analyzed by the RT-PCR method. Whether anti-HLA-F antibodies were present in sera from cancer patients was investigated through Western blotting with recombinant HLA-F as an antigen.
Results:
Transcripts of the HLA-F gene were produced by various tumor cell lines. In addition, anti-HLA-F IgG was present in the sera derived from various types of cancer patients (26 positives / 42 tested), but not in the sera derived from healthy donors (0 / 20).
Conclusion:
Anti-HLA-F antibodies should be further investigated as possible diagnostic tumor markers.
