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Evaluation of two rapid assays for detecting Cryptosporidium parvum in calf feces
Janna L Muccio1, Daniel L Grooms, Linda S Mansfield
1Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Objective:
To evaluate 2 rapid, patient-side assays for detection of Cryptosporidium parvum in feces from neonatal calves with diarrhea.
Design:
Diagnostic test evaluation Sample Population-Fecal samples from 96 neonatal (1 to 30 days old) calves with diarrhea.
Procedure:
Results of the rapid assays were compared with results of microscopic examination of fecal smears that had been stained with diamant fuchsin stain.
Results:
One of the rapid assays correctly identified 56 of 62 (90%) fecal samples positive for C. parvum oocysts and 33 of 34 (97%) fecal samples negative for oocysts. The other assay correctly identified 53 of 62 (85%) fecal samples positive for oocysts and 33 of 34 (97%) fecal samples negative for oocysts.
Conclusions And Clinical Relevance:
Results suggest that these 2 rapid assays are accurate when used to detect C. parvum in fecal samples from neonatal calves with diarrhea.
