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Related Experiment Videos

Microfluidic separation and gateable fraction collection for mass-limited samples.

Joseph J Tulock1, Mark A Shannon, Paul W Bohn

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Analytical Chemistry
|November 2, 2004
PubMed
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This study introduces a microfluidic system for precise separation and collection of attomole samples. It enables automated transfer of analytes, advancing microchip-based analyses for mass-limited samples.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Biotechnology

Background:

  • Integrating multiple analytical processes into microfluidic devices is crucial for advanced microchip-based analyses.
  • Handling minute sample quantities (attomole levels) presents significant challenges in microfluidic separations.

Purpose of the Study:

  • To describe a novel microfluidic system capable of preparative separations and intelligent fraction collection of attomole quantities.
  • To demonstrate automated analyte transfer for post-separation processing of mass-limited samples.

Main Methods:

  • A microfluidic device featuring a main electrophoresis channel interconnected to two vertically displaced channels via a nanocapillary array membrane.
  • Electrical bias applied across the nanocapillary array for controlled sample injection and analyte collection.

Related Experiment Videos

  • A fluorescence detector triggering automated voltage adjustments for near-quantitative analyte transfer.

    • Main Results:

    • Successful separation and automated, intelligent fraction collection of FITC-labeled amino acids (Arg, Gln, Gly) at attomole levels.
    • Near-quantitative transfer of collected analytes to a second fluidic layer.
    • Demonstration of precise sample manipulation capabilities for mass-limited samples.

    Conclusions:

    • The developed microfluidic system effectively achieves preparative separations and automated fraction collection for attomole samples.
    • This technology facilitates diverse post-separation processing events, expanding the utility of microfluidic devices for sensitive analyses.