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Published on: October 21, 2011
Effect of six-and ten-day-old chick embryo amniotic fluid on development of two-cell mouse embryos
Fariba Esmaili1, Mojtaba Rezazadeh Valojerdi
1Department of Anatomy, School of Medical Science, Tarbiat Modarres University, Tehran, Iran.
Insights
Chick embryo amniotic fluid (CEAF) effectively supports early mouse embryo development. Both six- and ten-day-old CEAF promoted embryo development to the blastocyst stage and hatching, indicating its potential as a culture medium.
Area of Science:
- Developmental Biology
- Embryology
- Reproductive Science
Background:
- Optimizing in vitro culture media is crucial for successful embryo development.
- Chick embryo amniotic fluid (CEAF) has potential as a biological supplement for mammalian embryo culture.
Purpose of the Study:
- To evaluate the effect of six- and ten-day-old CEAF on the developmental potential of two-cell mouse embryos.
- To compare the efficacy of CEAF as a supplement versus a natural medium for embryo culture.
Main Methods:
- Two-cell mouse embryos were cultured in various concentrations of heat-inactivated and active six-day-old CEAF (6-AF) and ten-day-old CEAF (10-AF).
- Embryo development rates to the blastocyst stage and hatching rates were monitored daily.
- Experimental groups were statistically compared against a control group using Ham's F-10 medium with human serum albumin.
Main Results:
- Embryos cultured in all CEAF groups showed significantly higher rates of development to the blastocyst stage compared to the control.
- Hatching rates increased from day 3 to day 5 of cultivation in all CEAF-treated groups.
- The developmental potential of embryos was comparable between six- and ten-day-old CEAF.
Conclusions:
- Chick embryo amniotic fluid (CEAF) can effectively support the development of two-cell mouse embryos in vitro.
- CEAF demonstrates potential as a valuable supplement or natural medium for improving mammalian embryo culture outcomes.
Abstract:
The purpose of this study was to investigate the effect of six-and ten-day-old chick embryo amniotic fluid (CEAF) on the development of two-cell mouse embryos. Six- and ten-day-old CEAF (6-AF, 10-AF) were aspirated separately from the amniotic cavity and two experiments were performed. In the first experiment, two-cell mouse embryos were cultured in different supplements of heat-inactivated 6-AF, pure heat-inactivated 6-AF and pure active 6-AF. The second experiment was also carried out in the same manner using the 10-AF. The rate of the development of embryos in all groups were daily determined and statistically compared with that of control (Ham's F-10 supplemented with 5 mg/ml human serum albumin). During the cultivation period, more embryos reached to the blastocyst stage in all groups of CEAF compared to the control. The result hatched blastocysts embryos also increased from days 3 till 5 of cultivation in all groups of CEAF. The developmental potential of embryos appears to be almost identical in six- and ten-day-old CEAF. In conclusion, CEAF as a supplement or a natural medium could support the development of two-cell mouse embryos.
