Related Experiment Video
Updated: Jul 28, 2026
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
Published on: March 2, 2012
[Cell transplantation for a CSF-fistula. Experience with fibrin glue and fibroblasts]
G Wolf1, P K Plinkert, B Schick
1Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, Universitätskliniken des Saarlandes. greggwolf@gmx.de
Background:
In the present study we investigated the culturing of fibroblasts on fibrin glue embedded suture nets, to analyse the possibility of using these components in a suture application technique for the closure of a CSF-fistula.
Results:
Placement of centrally perforated dura pieces on fibrin glue coated surfaces resulted in cellular migration from the dura borders into the defect, resulting in a complete cellular closure of the perforation. Inversion microscopic follow-up during culturing and the Alamar blue-essay found strong growth stimulation for oral mucosa fibroblasts on fibrin glue coated surfaces by insulin and FGF. Three-dimensional fibroblast growth was observed along the suture lines in the presence of fibrin glue.
Conclusions:
Fibrin glue is an attractive extracellular matrix for cellular migration from the dura which is suited to fibroblast culturing in suture nets. Our findings support the idea of achieving closure of cerebrospinal fluid fistulas by suture application of autologous fibroblasts and fibrin/thrombin preparations as a realistic future goal.
More Related Videos
09:56Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
Published on: July 27, 2014
06:15A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.
Published on: June 14, 2015