Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Site-specific strand bias in gene correction using single-stranded oligonucleotides.

Charlotte B Sørensen1, Anne-Margrethe Krogsdam, Marie S Andersen

  • 1Department of Human Genetics, University of Aarhus, Wilhelm Meyers Allé Bld. 240, 8000 Aarhus C, Denmark. cbs@humgen.au.dk

Journal of Molecular Medicine (Berlin, Germany)
|November 2, 2004
PubMed
Summary

Gene editing efficiency using single-stranded oligo-deoxyribonucleotides (ssODNs) depends on the target DNA strand. Surrounding sequences, not just transcription complexes, influence which strand yields higher correction frequencies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic landscape of the human vaginal microbiome is linked to host genetics and population of origin.

Nature genetics·2026
Same author

Dual human milk oligosaccharide-fibre utilisation is a selection cue for the weaning gut microbiome.

Nature communications·2026
Same author

Integrated Single-Cell and Spatial Analysis Reveals a Metabolic-Immune Axis Driving Aortic Dissection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Association Between Cardiovascular Polygenic Risk and White Matter Hyperintensities: An Observational Study From the UK Biobank.

Hypertension (Dallas, Tex. : 1979)·2026
Same author

<i>Faecalibacterium longum</i> alleviates high-fat diet-induced obesity and protects the intestinal epithelial barrier in mice.

Gut microbes reports·2026
Same author

Effects of hypocaloric wholegrain rye vs refined wheat diets on weight loss, cardiometabolic risk factors and gut microbiota: A 12-week randomized controlled trial.

Clinical nutrition (Edinburgh, Scotland)·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Editing Technologies

Background:

  • Targeted gene editing using single-stranded oligo-deoxyribonucleotides (ssODNs) is a powerful tool in molecular biology.
  • Previous studies suggested that targeting the non-transcribed strand of a gene typically results in higher editing efficiency due to potential interference with transcription complexes on the transcribed strand.

Purpose of the Study:

  • To investigate the correlation between oligonucleotide polarity and correction frequency in ssODN-mediated gene editing.
  • To determine if transcription complex occupancy is the sole factor influencing strand bias in gene editing.

Main Methods:

  • Utilized ssODNs for targeted correction of two specific G>A mutations in an episomal beta-galactosidase gene.
  • Compared correction frequencies when using ssODNs complementary to either the transcribed or non-transcribed strand at two different mutation sites (positions 659 and 1567).

Related Experiment Videos

Main Results:

  • At position 659, the highest correction efficiency was achieved using an ssODN complementary to the transcribed strand.
  • Conversely, at position 1567, the highest correction frequency was observed with an ssODN complementary to the non-transcribed strand.
  • Demonstrated that factors beyond transcriptional complex occupancy, specifically the surrounding nucleotide sequences, significantly influence strand bias in ssODN-mediated gene editing.

Conclusions:

  • The strand bias observed in ssODN-mediated gene editing is not solely determined by the presence of transcription complexes.
  • Local sequence context surrounding the target site plays a crucial role in dictating the efficiency of gene correction.
  • Findings provide critical insights for designing more effective ssODNs for precise gene editing applications.