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Related Experiment Videos

Membrane tensiometer for heavy giant vesicles.

P-H Puech1, F Brochard-Wyart

  • 1Laboratoire PCC/UMR 168, Institut Curie, 11 rue P. et M. Curie, 75005 Paris, France. puech@mpi-cbg.de

The European Physical Journal. E, Soft Matter
|November 2, 2004
PubMed
Summary
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We present a simple method to measure giant vesicle membrane tension using vesicle flattening observed via reflection interference microscopy (RIM). This technique allows tension estimation for sedimented vesicles, even during evaporation or light-induced tensioning.

Area of Science:

  • Biophysics
  • Soft Matter Physics
  • Cell Biology

Background:

  • Membrane tension (sigma) is crucial for giant vesicle adhesion.
  • Traditional micropipette methods for measuring membrane tension are delicate and often require prior knowledge of tension.
  • Existing methods for deducing tension from vesicle profiles are limited to very low tensions.

Purpose of the Study:

  • To develop a simple method for estimating membrane tension in heavy vesicles sedimented near a surface.
  • To apply this method to study vesicle flattening during solution evaporation and light-induced tensioning.

Main Methods:

  • Utilizing reflection interference microscopy (RIM) to observe vesicle behavior.
  • Measuring the size of the flat region of sedimented vesicles to estimate membrane tension.

Related Experiment Videos

  • Applying the technique to monitor dynamic changes in vesicle tension.

    • Main Results:

    • A straightforward method for estimating membrane tension of sedimented giant vesicles was established.
    • The technique successfully tracked vesicle flattening during solution evaporation.
    • Light-induced tensioning of vesicles was monitored using this method.

    Conclusions:

    • The RIM-based method provides a practical approach to measure membrane tension in specific vesicle configurations.
    • This technique offers new possibilities for studying dynamic membrane tension changes in biological and artificial systems.